Anti-Lipoprotein Lipase Antibody [5D2] - BSA and Azide free (A279436)

Anti-Lipoprotein Lipase Antibody [5D2] - BSA and Azide free

Mouse monoclonal [5D2] antibody to Lipoprotein Lipase.

This antibody recognises an epitope within amino acids 380-410 of lipoprotein lipase (LPL), a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues.A deficiency of LPL can result in hypertriglyceridemia, and LPL plays a critical role in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions. Mouse anti Lipoprotein lipase antibody, clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions. Mouse anti Lipoprotein lipase antibody, clone 5D2 inhibits the activity of human LPL.

WB, ELISA, IP, Functional Assays

ELISA: 1:500 - 1:5,000

Bovine, Rat, Human, Chicken, Guinea Pig

This antibody does not cross react with Mouse

Purified bovine milk lipoprotein lipase.

Mouse

Monoclonal

5D2

IgG1

1 mg/ml

Approximately 53 kDa using partially purified LPL from postheparin plasma.

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti lipoprotein lipase antibody, clone 5D2 recognizes an epitope within amino acids 380-410 of lipoprotein lipase (LPL), a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues.A deficiency of LPL can result in hypertriglyceridemia, and LPL plays a critical role in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions. Mouse anti Lipoprotein lipase antibody, clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions. Mouse anti Lipoprotein lipase antibody, clone 5D2 inhibits the activity of human LPL (Chang et al. 1998).

EC:3.1.1.32, LIPD, LPL, Phospholipase A1