Anti-LIS1 Antibody [ARC2075] (A307979)

Name: Anti-LIS1 Antibody [ARC2075]
Brand: Antibodies.com
SKU: A307979
Price: 340.0 GBP
Availability: InStock

Enlarge Image

This product is discontinued

Anti-LIS1 Antibody [ARC2075] (A307979) has been discontinued and is no longer available.

View all LIS1 Antibodies.

Specifications

Name

Anti-LIS1 Antibody [ARC2075]

Description

Rabbit monoclonal [ARC2075] antibody to LIS1.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LIS1 (P43034).

Sequence

MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDVNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2075

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

47 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

LIS-1, Lissencephaly-1 protein, MDCR, MDS, PAF acetylhydrolase 45 kDa subunit, PAF-AH 45 kDa subunit, PAF-AH alpha, PAFAH alpha, PAFAH1B1, PAFAHA, Platelet-activating factor acetylhydrolase IB subunit beta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

Enlarge Image

Western Blot - Anti-LIS1 Antibody [ARC2075] (A307979)

Western blot analysis of extracts of various cell lines, using Anti-LIS1 Antibody (A307979) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit. Exposure time: 180s.

Enlarge Image

Immunofluorescence - Anti-LIS1 Antibody [ARC2075] (A307979)

Immunofluorescence analysis of C6 cells using Anti-LIS1 Antibody (A307979) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Immunofluorescence - Anti-LIS1 Antibody [ARC2075] (A307979)

Immunofluorescence analysis of NIH-3T3 cells using Anti-LIS1 Antibody (A307979) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Immunofluorescence - Anti-LIS1 Antibody [ARC2075] (A307979)

Immunofluorescence analysis of U-2 OS cells using Anti-LIS1 Antibody (A307979) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).