Anti-LSM4 Antibody (A88309)

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This product is discontinued

Anti-LSM4 Antibody (A88309) has been discontinued and is no longer available.

View all LSM4 Antibodies.

Specifications

Name

Anti-LSM4 Antibody

Description

Rabbit polyclonal antibody to LSM4.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:20-1:50

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of human LSM4 (NP_036453.1).

Sequence

MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVAKGRGRGGLQQQKQQKGRGMGGAGRGVFGGRGRGGIPGTGRGQPEKKPGRQAGKQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Glycine-rich protein, GRP, U6 snRNA-associated Sm-like protein LSm4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-LSM4 Antibody (A88309)

Western blot analysis of extracts of various cell lines, using Anti-LSM4 Antibody (A88309) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 5s.

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Immunohistochemistry - Anti-LSM4 Antibody (A88309)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-LSM4 Antibody (A88309) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-LSM4 Antibody (A88309)

Immunofluorescence analysis of HeLa cells using Anti-LSM4 Antibody (A88309) at a dilution of 1:50. DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-LSM4 Antibody (A88309)

Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 1µg of Anti-LSM4 Antibody (A88309). This Western blot was performed on the immunoprecipitate using Anti-LSM4 Antibody (A88309) at a dilution of 1:1000.