Anti-Ly6c Antibody [ER-MP20] (PE) (A280371)

Anti-Ly6c Antibody [ER-MP20] (PE)

Rat monoclonal [ER-MP20] antibody to Ly6c (PE).

This antibody recognises mouse Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow. Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2.

Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Mouse

BALB/c macrophage precursor cell hybrids.

Rat

Monoclonal

ER-MP20

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse Ly-6C antibody, clone ER-MP20 recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with clone ER-MP12 in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenen et al. 1990).Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designated LY6C2. The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2.

This product is for research use only. It is not intended for diagnostic or therapeutic use.