Mouse monoclonal [CC98] antibody to LY75 / DEC-205 (FITC).
Specificity
This antibody recognises the bovine CD205 cell surface antigen, a ~210-220 kDa molecule expressed by T cells that are CD2+ve but not WC1+ve. CD205 is also expressed by B cells, and weakly stains B cell follicles.Bovine CD205 has previously been described as the WC6 antigen. Dendritic cells (veiled cells) in afferent lymph are strong expressors of CD205 as are dendritic cells in various other tissues.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Bovine, Sheep
Host
Mouse
Clonality
Monoclonal
Clone ID
CC98
Isotype
IgG2b
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Bovine CD205 antibody, clone CC98 recognizes the bovine CD205 cell surface antigen, a ~210-220 kDa molecule expressed by T cells that are CD2+ve but not WC1+ve. CD205 is also expressed by B cells, and weakly stains B cell follicles.Bovine CD205 has previously been described as the WC6 antigen (Gliddon et al. 2004).Dendritic cells (veiled cells) in afferent lymph are strong expressors of CD205 as are dendritic cells in various other tissues.