Mouse monoclonal [IL-A114] antibody to LY75 / DEC-205 (PE).
Specificity
This antibody recognises bovine CD205 (also known as DEC205) and originally described as BoWC6. CD205 is a cell surface antigen with an approximate molecular weight of ~210-220 kDa. CD205 is expressed on afferent lymph dendritic cells (veiled cells), B cells, a subset of T cells and by cortical and medullary thymocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Bovine, Sheep
Host
Mouse
Clonality
Monoclonal
Clone ID
IL-A114
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Bovine CD205 antibody, clone IL-A114 recognizes bovine CD205 (also known as DEC205) and originally described as BoWC6. CD205 is a cell surface antigen with an approximate molecular weight of ~210-220 kDa. CD205 is expressed on afferent lymph dendritic cells (veiled cells), B cells, a subset of T cells and by cortical and medullary thymocytes.
Figure A. Anti-CD32 Antibody [CCG39] (FITC) (A280075) and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Anti-CD32 Antibody [CCG39] (FITC) (A280075) and Anti-LY75 / DEC-205 Antibody [IL-A114] (PE) (A280710). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Figure A. Anti-LY75 / DEC-205 Antibody [IL-A114] (PE) (A280710) and Mouse IgG2a (FITC) - Isotype Control (A282454). Figure B. Anti-LY75 / DEC-205 Antibody [IL-A114] (PE) (A280710) and Anti-CD32 Antibody [CCG39] (FITC) (A280075). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
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