Anti-LysRS Antibody [ARC1765] (A308875)

Name: Anti-LysRS Antibody [ARC1765]
Brand: Antibodies.com
SKU: A308875
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1765] antibody to LysRS for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-LysRS Antibody [ARC1765]

Description

Rabbit monoclonal [ARC1765] antibody to LysRS.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 500-597 of human LysRS/KARS (Q15046).

Sequence

TELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1765

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

KARS, KARS1, KIAA0070, Lysine--tRNA ligase, Lysyl-tRNA synthetase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-LysRS Antibody [ARC1765] (A308875)

Western blot analysis of extracts of various cell lines, using Anti-LysRS Antibody [ARC1765] (A308875) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-LysRS Antibody [ARC1765] (A308875)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-LysRS Antibody [ARC1765] (A308875) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-LysRS Antibody [ARC1765] (A308875)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-LysRS Antibody [ARC1765] (A308875) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-LysRS Antibody [ARC1765] (A308875)

Immunofluorescence analysis of C6 cells using Anti-LysRS Antibody [ARC1765] (A308875) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).