Anti-Macrophages Antibody [BA4D5] (A279779)

Anti-Macrophages Antibody [BA4D5]

Mouse monoclonal [BA4D5] antibody to Macrophages.

This antibody recognises porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.Some expression has also been observed on peripheral blood lymphocytes.The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.It is believed that clone BA4D5 may be specific for porcine CD68 although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.

WB, IP, Flow Cytometry, IHC-Fr, IF

FC 1: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Porcine

Porcine alveolar macrophages.

Mouse

Monoclonal

BA4D5

IgG2b

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig Macrophages antibody, clone BA4D5 recognizes porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.Some expression has also been observed on peripheral blood lymphocytes.The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.It is believed that clone BA4D5 may be specific for porcine CD68 (Poulsen et al. 2016) although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.