Mouse monoclonal [15-2] antibody to Mannose Receptor (PE-Cyanine 7).
Specificity
This antibody, also known as Clone MR15-2, recognizes an extracellular epitope of CD206, a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Purified human mannose receptor.
Host
Mouse
Clonality
Monoclonal
Clone ID
15-2
Isotype
IgG1
Light Chains
kappa
Conjugate
PE-Cyanine 7
Excitation: 565nm, Emission: 778nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
162-175 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
bA541I19.1, C type lectin domain family 13 member D, C-type lectin domain family 13 member D, CD 206, CD206, CD206 antigen, CLEC13D, CLEC13DL, Macrophage mannose receptor, Macrophage mannose receptor 1, Macrophage mannose receptor 1 like protein 1, Mannose receptor C type 1, Mannose receptor C type 1 like 1, MMR, MRC 1, MRC1, MRC1L1, MRC1_HUMAN, OTTHUMP00000045206
Flow cytometric analysis of human stimulated monocytes (GM-CSF + IL-4) stained with Anti-CD206 Antibody [15-2] (PE-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).
Flow cytometric analysis of human stimulated monocytes (GM-CSF + IL-4) stained using Anti-CD206 Antibody [15-2] (PE-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension) and Anti-CD11c Antibody [BU15] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
Separation of human CD206 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis of human stimulated monocytes (GM-CSF + IL-4) using Anti-CD206 Antibody [15-2] (PE-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).