Western Blot - Anti-MAPK6/ERK3 (phospho Ser189) Antibody (A16417)
Western blot analysis of extracts of 293 cells, using Anti-MAPK6/ERK3 (phospho Ser189) Antibody (A16417) at 1:1,000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
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