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Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778)

Western Blot - Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778) - Antibodies.com Western Blot - Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778) - Antibodies.com Immunofluorescence - Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778) - Antibodies.com
$255
Product Datasheet

Mouse monoclonal [AMC0522] antibody to MBP Tag for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-MBP Tag Antibody [AMC0522] (HRP)
Description
Mouse monoclonal [AMC0522] antibody to MBP Tag.
Applications
WB
Dilutions
WB: 1:2,000-1:10,000
Reactivity
Species Independent
Immunogen
A synthetic peptide of MBP-Tag.
Host
Mouse
Clonality
Monoclonal
Clone ID
AMC0522
Isotype
IgG1
Light Chains
kappa
Conjugate

HRP

Purification
Affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778) - Antibodies.comEnlarge Image

Western Blot - Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778)

Western blot analysis of extracts of various cell lines, using Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778) - Antibodies.comEnlarge Image

Western Blot - Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778)

Western blot analysis of extracts of C6 cells, using Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunofluorescence - Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778)

Immunofluorescence analysis of U-2 OS cells using Anti-MBP Tag Antibody [AMC0522] (HRP) (A309778) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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