Anti-MCM5 Antibody (A14837)

Name: Anti-MCM5 Antibody
Brand: Antibodies.com
SKU: A14837
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to MCM5 for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-MCM5 Antibody

Description

Rabbit polyclonal antibody to MCM5.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:1,000-1:3,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).

Sequence

MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEVLQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLTNIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CDC46, CDC46 homolog, DNA replication licensing factor MCM5, P1-CDC46

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-MCM5 Antibody (A14837)

Western blot analysis of extracts of various cell lines, using Anti-MCM5 Antibody (A14837) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunofluorescence - Anti-MCM5 Antibody (A14837)

Immunofluorescence analysis of U-2 OS cells using Anti-MCM5 Antibody (A14837) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-MCM5 Antibody (A14837)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-MCM5 Antibody (A14837). This Western blot was performed on the immunoprecipitate using Anti-MCM5 Antibody (A14837) at a dilution of 1:1000.

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