Anti-MED4 Antibody [ARC2528] (A306502)

Name: Anti-MED4 Antibody [ARC2528]
Brand: Antibodies.com
SKU: A306502
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC2528] antibody to MED4 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-MED4 Antibody [ARC2528]

Description

Rabbit monoclonal [ARC2528] antibody to MED4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human MED4 (Q9NPJ6).

Sequence

KYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2528

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

36 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Activator-recruited cofactor 36 kDa component, ARC36, DRIP36, Mediator complex subunit 4, Mediator of RNA polymerase II transcription subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, VDRIP, Vitamin D3 receptor-interacting protein complex 36 kDa component

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-MED4 Antibody [ARC2528] (A306502)

Western blot analysis of extracts of various cell lines, using Anti-MED4 Antibody [ARC2528] (A306502) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-MED4 Antibody [ARC2528] (A306502)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-MED4 Antibody [ARC2528] (A306502) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-MED4 Antibody [ARC2528] (A306502)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-MED4 Antibody [ARC2528] (A306502) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-MED4 Antibody [ARC2528] (A306502)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-MED4 Antibody [ARC2528] (A306502) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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