Rabbit polyclonal antibody to MEK1 / MEK2 (Phospho-Ser217 / Ser221)
Specificity
The antibody detects endogenous level of MEK1 / MEK2 only when phosphorylated at serine 217 / 221.
Applications
IHC, WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 217 / 221 (I-D-S(p)-M-A) derived from Human MEK1 / MEK2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/MEK2 (Phospho-Ser217/Ser221) Antibody #11205 (left) or the same antibody preincubated with blocking peptide (right).
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