Western Blot - Anti-MEK2 (phospho Thr394) Antibody (A16450)
Western blot analysis of extracts from HepG2 cells using Anti-MEK2 (phospho Thr394) Antibody (A16450). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-MEK2 (phospho Thr394) Antibody (A16450). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.