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Anti-MEK4 / MKK4 Antibody (A10211)

Western Blot - Anti-MEK4 / MKK4 Antibody (A10211) - Antibodies.com Western Blot - Anti-MEK4 / MKK4 Antibody (A10211) - Antibodies.com
This product is discontinued

Anti-MEK4 / MKK4 Antibody (A10211) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-MEK4 / MKK4 Antibody
Description
Rabbit polyclonal antibody to MEK4 / MKK4.
Applications
WB, IP
Dilutions
WB: 1:500-1:1,000, IP: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-404 of human MAP2K4 (NP_003001.1).
Sequence
LATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
44 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-MEK4 / MKK4 Antibody (A10211) - Antibodies.comEnlarge Image

Western Blot - Anti-MEK4 / MKK4 Antibody (A10211)

Western blot analysis of HeLa, using Anti-MEK4 / MKK4 Antibody (A10211) at 1:700 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-MEK4 / MKK4 Antibody (A10211) - Antibodies.comEnlarge Image

Western Blot - Anti-MEK4 / MKK4 Antibody (A10211)

Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg of Anti-MEK4 / MKK4 Antibody (A10211). This Western blot was performed on the immunoprecipitate using Anti-MEK4 / MKK4 Antibody (A10211) at a dilution of 1:1000.