Western Blot - Anti-MEK4/MKK4 (phospho Ser257 + Thr261) Antibody (A11019)
Western blot analysis of extracts of HeLa cells, using Anti-MEK4/MKK4 (phospho Ser257 + Thr261) Antibody (A11019) at 1:1,000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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