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Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45] (A250932)

Immunohistochemistry - Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45] (A250931) - Antibodies.com Western Blot - Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45] (A250932) - Antibodies.com
$545
Product Datasheet

Mouse monoclonal [A103 + T311 + HMB45] antibody to Melanoma Marker (MART-1 + Tyrosinase + gp100) for WB and IHC-P.

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Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45]
Description
Mouse monoclonal [A103 + T311 + HMB45] antibody to Melanoma Marker (MART-1 + Tyrosinase + gp100).
Specificity
This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Applications
WB, IHC-P
Dilutions
WB: 2-4 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Clone A103: Recombinant human MART-1 protein. Clone T311: Recombinant tyrosinase protein. Clone HMB45: Extract of pigmented melanoma metastases from lymph nodes.
Host
Mouse
Clonality
Oligoclonal
Clone ID
A103 + T311 + HMB45
Isotype
IgG1, kappa + IgG2a, kappa + IgG1, kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
20-22 kDa (doublet) (MART) / 70-80 kDa (Tyrosinase) / 26-100 kDa (gp100)
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes

Please note, this product is an oligoclonal antibody; it is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies not only have the specificity and batch-to-batch consistency of a monoclonal antibody, but they also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

This oligoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45] - BSA and Azide free (A254112).

Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Immunohistochemistry - Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45] (A250931) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45] (A250932)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human melanoma using Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45].
Western Blot - Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45] (A250932) - Antibodies.comEnlarge Image

Western Blot - Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45] (A250932)

Western blot analysis of COLO-38 cell lysate using Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45].
Publishing research using Anti-Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45] (A250932)? Please let us know so that we can list the citation on this page.