Rabbit polyclonal antibody to MER / SKY (Phospho-Tyr749 / 681)
Specificity
The antibody detects endogenous levels of MER / SKY only when phosphorylated at tyrosine 749 / 681.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 749 / 681(K-I-Y(p)-S-G) derived from Human MER / SKY .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
MERTK, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, Tyrosine-protein kinase Mer
Western blot analysis of extracts from 3T3 cells treated with EGF using MER/SKY (Phospho-Tyr749/681) Antibody #11740.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody #11740 (left)or the same antibody preincubated with blocking peptide (right).
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