Anti-METTL16 Antibody (A91255)

Name: Anti-METTL16 Antibody
Brand: Antibodies.com
SKU: A91255

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This product is discontinued

Anti-METTL16 Antibody (A91255) has been discontinued and is no longer available.

View all METTL16 Antibodies.

Specifications

Name

Anti-METTL16 Antibody

Description

Rabbit polyclonal antibody to METTL16.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human METTL16 (NP_076991.3).

Sequence

RIQGVPKVTYTEFCQGRTMRWALAWSFYDDVTVPSPPSKRRKLEKPRKPITFVVLASVMKELSLKASPLRSETAEGIVVVTTWIEKILTDLKVQHKRVPCGKEEVSLFLTAIENSWIHLRRKKRERVRQLREVPRAPEDVIQALEEKKPTPKESGNSQELARGPQERTPCGPALREGEAAAVEGPCPSQESLSQEENPEPTEDERSEEKGGVEVLESCQGSSNGAQDQEASEQFGSPVAERGKRLPGVAGQYLFKCLINVKKEVDDALVEMHWVEGQNRDLMNQLCTYIRNQIFRLVAVN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

adenine-(43)-N(6))-methyltransferase, EC:2.1.1.348, Methyltransferase 10 domain-containing protein, Methyltransferase-like protein 16, METT10D, N6-adenosine-methyltransferase METTL16, RNA N6-adenosine-methyltransferase METTL16, U6 small nuclear RNA

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-METTL16 Antibody (A91255)

Western blot analysis of various lysates, using Anti-METTL16 Antibody (A91255) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunofluorescence - Anti-METTL16 Antibody (A91255)

Immunofluorescence analysis of C6 cells using Anti-METTL16 Antibody (A91255) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-METTL16 Antibody (A91255)

Immunofluorescence analysis of NIH/3T3 cells using Anti-METTL16 Antibody (A91255) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-METTL16 Antibody (A91255)

Immunofluorescence analysis of U-2 OS cells using Anti-METTL16 Antibody (A91255) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).