Anti-MHC Class I H-2b/D/P/Q/w16 Antibody [ER-HR52] (A280358)

Anti-MHC Class I H-2b/D/P/Q/w16 Antibody [ER-HR52]

Rat monoclonal [ER-HR52] antibody to MHC Class I H-2b/D/P/Q/w16.

This antibody recognises a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells. Clone ER-HR52 specifically recognises mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.

Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Mouse

Adherent FI (CBAXBL) bone marrow stromal cells.

Rat

Monoclonal

ER-HR52

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells. Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.