Anti-MHC Class I Monomorphic Antibody [CVS22] (FITC)
Description
Mouse monoclonal [CVS22] antibody to MHC Class I Monomorphic (FITC).
Specificity
This antibody recognises monomorphic equine MHC Class I and was classified at the International Equine Leucocyte Antigen Workshop. MHC class I is expressed by all nucleated cells.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this complex is referred to as the equine leukocyte antigen (ELA) region.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Horse
Immunogen
Equine leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
CVS22
Isotype
IgG2a
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Horse MHC Class I Monomorphic antibody, clone CVS22 recognizes monomorphic equine MHC Class I and was classified at the International Equine Leucocyte Antigen Workshop. MHC class I is expressed by all nucleated cells.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this complex is referred to as the equine leukocyte antigen (ELA) region.
Flow Cytometry - Anti-MHC Class I Monomorphic Antibody [CVS22] (FITC) (A280169)
Figure A. Anti-CD11a/CD18 Antibody [CVS9] (PE) (A280187) and Mouse IgG1 (FITC) - Isotype Control (A282453). Figure B. Anti-CD11a/CD18 Antibody [CVS9] (PE) (A280187) and Anti-MHC Class I Monomorphic Antibody [CVS22] (FITC) (A280169). All experiments performed on red cell lysed horse blood gated on lymphocytes in the presence of 10% horse serum. Data acquired on the ZE5 Cell Analyzer.
Publishing research using Anti-MHC Class I Monomorphic Antibody [CVS22] (FITC) (A280169)? Please let us know so that we can list the citation on this page.