Mouse monoclonal [OX-27] antibody to MHC Class I RT1Ac (PE).
Specificity
This antibody recognises a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
PHA activated rat lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-27
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 recognizes a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
