Anti-MHC Class II Monomorphic Antibody [YKIX334.2] (A282051)

Anti-MHC Class II Monomorphic Antibody [YKIX334.2]

Rat monoclonal [YKIX334.2] antibody to MHC Class II Monomorphic.

This antibody recognises a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti Dog MHC Class II immunoprecipitates an antigen of ~32/34 kDa and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.

Flow Cytometry, IHC-Fr, IP

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Canine

This antibody does not cross react with Hooded Seal

Con A activated canine peripheral blood cells.

Rat

Monoclonal

YKIX334.2

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti Dog MHC Class II immunoprecipitates an antigen of ~32/34 kDa and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.