Anti-MHC Class II RT1B Antibody [OX-6] (A281478)

Anti-MHC Class II RT1B Antibody [OX-6]

Mouse monoclonal [OX-6] antibody to MHC Class II RT1B.

This antibody recognises a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.Rat MHC Class II RT1B antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region. Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster and Williams 1979 and Male et al. 1987). Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 is routinely tested in flow cytometry on rat splenocytes.

IHC-P, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 - 1:100, IHC-P: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat, Mouse

Rat Thymocyte Membrane Glycoproteins.

Mouse

Monoclonal

OX-6

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.Rat MHC Class II RT1B antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male et al. 1987).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster and Williams 1979 and Male et al. 1987).Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 is routinely tested in flow cytometry on rat splenocytes.