Western blot analysis of various lysates, using Anti-MIRO1 Antibody (A309670) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western blot analysis of Rat testis, using Anti-MIRO1 Antibody (A309670) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western blot analysis of extracts from wild type(WT) and RHOT1/MIRO1 knockdown (KD) 293T(KD) cells, using Anti-MIRO1 Antibody (A309670) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using Anti-MIRO1 Antibody (A309670) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
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