Rabbit polyclonal antibody to MITF (Phospho-Ser180 / 73)
Specificity
The antibody detects endogenous level of MITF only when phosphorylated at Serine 180 / 73.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 180(P-N-S(p)-P-M) / Serine 73(Q-E-R(p)-R-E) derived from Human MITF.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
bHLHe32, Class E basic helix-loop-helix protein 32, Microphthalmia-associated transcription factor
Western blot analysis of extracts from Mouse brain tissue using MITF (Phospho-Ser180/73) Antibody #12018.The lane on the right is treated with the antigen-specific peptide.
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