Anti-Mitofilin Antibody (A14169)

Name: Anti-Mitofilin Antibody
Brand: Antibodies.com
SKU: A14169
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Mitofilin for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Mitofilin Antibody

Description

Rabbit polyclonal antibody to Mitofilin.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 120-270 of human IMMT (NP_006830.2).

Sequence

ESKQPASQLQKQKGDTPASATAPTEAAQIISAAGDTLSVPAPAVQPEESLKTDHPEIGEGKPTPALSEEASSSSIRERPPEEVAARLAQQEKQEQVKIESLAKSLEDALRQTASVTLQAIAAQNAAVQAVNAHSNILKAAMDNSEIAGEKK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cell proliferation-inducing gene 4/52 protein, HMP, IMMT, MIC60, MICOS complex subunit MIC60, MINOS2, Mitochondrial inner membrane protein, p87/89

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Mitofilin Antibody (A14169)

Western blot analysis of extracts of various cell lines, using Anti-Mitofilin Antibody (A14169) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-Mitofilin Antibody (A14169)

Western blot analysis of extracts of Mouse brain, using Anti-Mitofilin Antibody (A14169) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunofluorescence - Anti-Mitofilin Antibody (A14169)

Confocal immunofluorescence analysis of U-2 OS cells using Anti-Mitofilin Antibody (A14169) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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