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Recombinant Anti-MMAE Antibody [8C4] - BSA and Azide free (A324885)

ELISA - Anti-MMAE Antibody [8C4] - BSA and Azide free (A324885) - Antibodies.com
Recombinant Antibody
$390
Product Datasheet

Recombinant rabbit monoclonal [8C4] antibody to MMAE for ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-MMAE Antibody [8C4] - BSA and Azide free
Description
Recombinant rabbit monoclonal [8C4] antibody to MMAE.
Applications
ELISA
Dilutions
ELISA: 1:5,000-10,000
Host
Rabbit
Clonality
Monoclonal
Clone ID
8C4
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%-8% Trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

ELISA - Anti-MMAE Antibody [8C4] - BSA and Azide free (A324885) - Antibodies.comEnlarge Image

Anti-MMAE Antibody [8C4] - BSA and Azide free (A324885)

ELISA plates were pre-coated with IgG-MMAE at 0.2µg/per well. Serial diluted Anti-MMAE Antibody [8C4] - BSA and Azide free (A324885) solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-MMAE Antibody [8C4] - BSA and Azide free (A324885) binding with IgG-MMAE is 1.356ng/ml.
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