Western blot analysis of extracts of various cell lines, using Anti-MMP13 Antibody (A80887) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western blot analysis of various lysates, using Anti-MMP13 Antibody (A80887) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from wild type(WT) and MMP13 knockdown (KD) 293T(KD) cells, using Anti-MMP13 Antibody (A80887) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of C6 cells using Anti-MMP13 Antibody (A80887) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of L929 cells using Anti-MMP13 Antibody (A80887) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-MMP13 Antibody (A80887) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
