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Anti-mNeonGreen Antibody (A329616)

Western Blot - Anti-mNeonGreen Antibody (A329616) - Antibodies.com Immunofluorescence - Anti-mNeonGreen Antibody (A329616) - Antibodies.com
$515
Product Datasheet

Rabbit polyclonal antibody to mNeonGreen for WB, ICC/IF and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-mNeonGreen Antibody
Description
Rabbit polyclonal antibody to mNeonGreen.
Applications
WB, ICC/IF, ELISA
Positive Control
293T.
Dilutions
WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200
Reactivity
Species Independent
Immunogen
A recombinant fusion protein corresponding to amino acids 10-236 of Branchiostoma lanceolatum mNeonGreen.
Sequence
MGRDLYDDDDKDPLEMVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
30 kDa
Predicted MW
30 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-mNeonGreen Antibody (A329616) - Antibodies.comEnlarge Image

Western Blot - Anti-mNeonGreen Antibody (A329616)

mNeonGreen expression in lysates from wild type (WT) and 293T cells transfected with mNeonGreen analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-mNeonGreen Antibody (A329616) at a dilution of 1:2000 and detected with chemiluminescence.
Immunofluorescence - Anti-mNeonGreen Antibody (A329616) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-mNeonGreen Antibody (A329616)

mNeonGreen expression in 293F-6Xhis-mNeonGreen-S-tag(C) and 293F cells analyzed by immunofluorescence. Staining was performed with Anti-mNeonGreen Antibody (A329616) at a dilution of 1:100 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
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