Recombinant humanized monoclonal antibody to NKG2A.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG4
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 145 kDa.
Purity
> 90% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
CD159 antigen-like family member A, CD159a, KLRC1, NK cell receptor A, NKG2-A/B-activating NK receptor, NKG2-A/NKG2-B type II integral membrane protein
SDS-PAGE analysis of Monalizumab Biosimilar - Anti-NKG2A Antibody - Low endotoxin, Azide free (A323588) under reducing (R) conditions confirms that the purity of this antibody is greater than 90%.
SEC-HPLC analysis of Monalizumab Biosimilar - Anti-NKG2A Antibody - Low endotoxin, Azide free (A323588) confirms that the purity of this antibody is greater than 95%.
Human NKG2A/CD94 HEK293 cells were stained with Monalizumab Biosimilar - Anti-NKG2A Antibody - Low endotoxin, Azide free (A323588) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.0409 µg/ml.
Monalizumab Biosimilar - Anti-NKG2A Antibody - Low endotoxin, Azide free (A323588) FACS blocking was evaluated using human NKG2A/CD94 HEK293 cells. The IC50 was approximately 0.2162 nM.
Monalizumab Biosimilar - Anti-NKG2A Antibody - Low endotoxin, Azide free (A323588) Luciferase Assay was evaluated using human NKG2A/CD94 HEK293. The EC50 was approximately 0.5236 nM.
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