Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1 x 106 cells or 100µl whole blood.
Host
Mouse
Clonality
Monoclonal
Isotype
IgM
Conjugate
Unconjugated
Purification
Ammonium sulphate precipiation of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at 4°C.
General Notes
Mouse IgM negative control antibody is designed for use as a negative control reagent to assess the level of non-specific binding of mouse IgM monoclonal antibodies to human cells.This antibody recognizes a cell surface protein on rat cells and is therefore not suitable as an isotype control for work on this species.
Flow Cytometry - Mouse IgM - Isotype Control (A284020)
Figure A. Anti-CD3 epsilon Antibody [PPT3] (FITC) (A281589) and Mouse IgM - Isotype Control (A284020) detected with Goat Anti-Mouse IgM Antibody (PE). Figure B. Anti-CD3 epsilon Antibody [PPT3] (FITC) (A281589) and Anti-CD45RC Antibody [MIL15] (A281202) detected with Goat Anti-Mouse IgM Antibody (PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
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