Western blotting analysis of mPlum fluorescent protein using Anti-mPlum Antibody (A285875) on lysates of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-mPlum polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for mPlum protein at approximately 30 kDa.
Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs labeling mPlum with Anti-mPlum Antibody (A285875), (concentration in sample 2 µg/ml, GAR APC).
Separation of HEK293T/17 cells co-transfected using Anti-mPlum Antibody (A285875), (concentration in sample 2 µg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mPlum/YFP cell suspension.
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