Western Blot - Anti-mtTFA Antibody [ARC51776] (A14389)
Western blot analysis of extracts of various cell lines, using Anti-mtTFA Antibody (A14389) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-mtTFA Antibody (A14389) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung using Anti-mtTFA Antibody (A14389) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-mtTFA Antibody (A14389) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U-2 OS cells using Anti-mtTFA Antibody (A14389) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Chromatin immunoprecipitation (ChIP) analysis of extracts of K-562 cells, using Anti-mtTFA Antibody (A14389) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
