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Anti-MTX1 / MTX Antibody (A306624)

Western Blot - Anti-MTX1 / MTX Antibody (A306624) - Antibodies.com Immunofluorescence - Anti-MTX1 / MTX Antibody (A306624) - Antibodies.com Immunofluorescence - Anti-MTX1 / MTX Antibody (A306624) - Antibodies.com
This product is discontinued

Anti-MTX1 / MTX Antibody (A306624) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-MTX1 / MTX Antibody
Description
Rabbit polyclonal antibody to MTX1 / MTX.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 186-435 of human MTX1 (NP_942584.2).
Sequence
LKVHKISNPWQSPSGTLPALRTSHGEVISVPHKIITHLRKEVHTFWIDTKNYVEVTRKWYAEAMPFPLNFFLPGRMQRQYMERLQLLTGEHRPEDEEELEKELYREARECLTLLSQRLGSQKFFFGDAPASLDAFVFSYLALLLQAKLPSGKLQVHLRGLHNLCAYCTHILSLYFPWDGAEVPPQRQTPAGPETEEEPYRRRNQILSVLAGLAAMVGYALLSGIVSIQRATPARAPGTRTLGMAEEDEEE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-MTX1 / MTX Antibody (A306624) - Antibodies.comEnlarge Image

Western Blot - Anti-MTX1 / MTX Antibody (A306624)

Western blot analysis of extracts of various cell lines, using Anti-MTX1 / MTX Antibody (A306624) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-MTX1 / MTX Antibody (A306624) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-MTX1 / MTX Antibody (A306624)

Immunofluorescence analysis of U2OS cells using Anti-MTX1 / MTX Antibody (A306624) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-MTX1 / MTX Antibody (A306624) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-MTX1 / MTX Antibody (A306624)

Confocal immunofluorescence analysis of U-2 OS cells using Anti-MTX1 / MTX Antibody (A306624) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).