Mouse monoclonal [C595 (NCRC48)] antibody to MUC1.
Specificity
This antibody recognises CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.recognises the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.
Applications
WB, IHC-P, Flow Cytometry, IHC-Fr, ELISA, IF
Dilutions
ELISA: 1:100 - 1:1,000
Reactivity
Human
Immunogen
Urinary MUC-1 mucin.
Host
Mouse
Clonality
Monoclonal
Clone ID
C595 (NCRC48)
Isotype
IgG3
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at 4°C.
General Notes
Mouse anti Human CD227 antibody, clone C595 (NCRC48) recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.
Synonyms
Breast carcinoma-associated antigen DF3, CA 15-3, Cancer antigen 15-3, Carcinoma-associated mucin, CD227, Episialin, H23AG, MUC-1, Mucin-1, PUM
Sandwich ELISA analysis of CD227 expression using Anti-MUC1 Antibody [C595 (NCRC48)] (A279960) as a capture reagent and biotinylated Anti-MUC1 Antibody [VU-4H5] (A281947) as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively
Sandwich ELISA analysis of CD227 expression using Anti-MUC1 Antibody [C595 (NCRC48)] (A279960) as a capture reagent and biotinylated Mouse anti Human CD227, clone 6A4 as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on a Microplate Absorbance Reader. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.
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