Rabbit polyclonal antibody to Myb (Phospho-Ser532)
Specificity
The antibody detects endogenous levels of Myb only when phosphorylated at serine 532.
Applications
IHC
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of serine 532 (V-E-S(p)-P-T) derived from Human Myb.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Myb (Phospho-Ser532) antibody #12145. The picture on the right is treated with the synthesized peptide.
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