Mouse monoclonal [9E10] antibody to Myc Tag (FITC).
Specificity
This antibody can be used to detect human c-Myc or the c-Myc tag.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-5 µg/ml.
Reactivity
Human, Epitope Tag
Immunogen
Synthetic peptide corresponding to the C-terminal region of human c-Myc.
Host
Mouse
Clonality
Monoclonal
Clone ID
9E10
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow Cytometry - Anti-Myc Tag Antibody [9E10] (FITC) (A85466)
Flow cytometry analysis of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (blue) using Anti-Myc Tag Antibody [9E10] (FITC) (A85466).
Flow Cytometry - Anti-Myc Tag Antibody [9E10] (FITC) (A85466)
Flow cytometry analysis (intracellular staining) of transfected LST-1-c-Myc in HEK-293 cells (red-filled) compared with nontransfected HEK-293 cells (black-dashed) using Anti-Myc Tag Antibody [9E10] (FITC) (A85466).
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