The mouse mAb detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected cells.
Applications
WB
Dilutions
Western blotting: 1:5000~1:10000
Immunogen
Peptide sequence around aa. 410-419 (EQKLISEEDL) derived from human c-Myc.
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Antibodies were produced from mice ascites by injecting mice with a monoclonal cell line which was fusioned by mouse spleen and SP2 / 0 myeloma cell. Spleen cells were isolated from mice by immunizing with synthetic peptide and KLH conjugates.
Concentration
1 mg/ml
Formulation
Supplied in mice ascites
Storage
Store at 4°C for short term use. Store at -20°C for long term preservation. Avoid freezing and thawing repeatedly.
