Western blot analysis of extracts of various cell lines, using MCCC2 antibody (A16027) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (A17345) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of various cells, using MCCC2 antibody (A16027) at 1:1,000 dilution ratio through one-step method. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (A17345) at 1:4000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.