All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 0.3ug/ml Lane 1: mouse heart muscle lysate. Lane 2: Recombinant Myoglobin at 10ugSecondary HRP labelled Goat polyclonal to Mouse IgG at 1/3000 dilution Predicted band size: 17kdObserved band size: 20kdAdditional bands at: 120KD (We are unsure as to the identity of this extra band.)
All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 1ug/ml Lane 1: Myoglobin transfected 293 cell lysate. Secondary HRP labelled Goat polyclonal to Mouse IgG at 1/3000 dilution Predicted band size: 17kdObserved band size: 20kdAdditional bands at: 23/25/40KD (We are unsure as to the identity of this extra band.)
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