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Anti-Myosin heavy chain Antibody [ARC1170] (A309012)

Western Blot - Anti-Myosin heavy chain Antibody [ARC1170] (A309012) - Antibodies.com Immunohistochemistry - Anti-Myosin heavy chain Antibody [ARC1170] (A309012) - Antibodies.com Immunofluorescence - Anti-Myosin heavy chain Antibody [ARC1170] (A309012) - Antibodies.com Immunofluorescence - Anti-Myosin heavy chain Antibody [ARC1170] (A309012) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1170] antibody to Myosin heavy chain for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Myosin heavy chain Antibody [ARC1170]
Description
Rabbit monoclonal [ARC1170] antibody to Myosin heavy chain.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1400 of human Myosin heavy chain (P12883).
Sequence
VAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1170
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
250 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-Myosin heavy chain Antibody [ARC1170] (A309012) - Antibodies.comEnlarge Image

Western Blot - Anti-Myosin heavy chain Antibody [ARC1170] (A309012)

Western blot analysis of extracts of various cell lines, using Anti-Myosin heavy chain Antibody [ARC1170] (A309012) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-Myosin heavy chain Antibody [ARC1170] (A309012) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Myosin heavy chain Antibody [ARC1170] (A309012)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Myosin heavy chain Antibody [ARC1170] (A309012) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Myosin heavy chain Antibody [ARC1170] (A309012) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Myosin heavy chain Antibody [ARC1170] (A309012)

Immunofluorescence analysis of rat heart using Anti-Myosin heavy chain Antibody [ARC1170] (A309012) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Myosin heavy chain Antibody [ARC1170] (A309012) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Myosin heavy chain Antibody [ARC1170] (A309012)

Immunofluorescence analysis of mouse heart using Anti-Myosin heavy chain Antibody [ARC1170] (A309012) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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