The Anti-N6-methyladenosine/m6A Antibody (A309558), (4µg) was tested in a Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer). Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin), Oligomer 5 - N6-methyladenosine (AUAACUGG-m6A-CCGAAUGG-Biotin), Oligomer 6 - unmodified adenosine (AUAACUGGACCGAAUGG-Biotin)
Dot Blot - Anti-N6-Methyladenosine/m6A Antibody [ARC5003-03] (A309558)
Anti-N6-Methyladenosine/m6A Antibody (A309558) was tested in dot blot against N6-methyladenosine (m6A) and unmodified adenosine. Oligomer 8: ATAACTGG-m6A-CCGAATGG Oligomer 9: ATAACTGGACCGAATGG Oligomer 10: AAAAAAAAAAAAAAAA-biotin.
Immunofluorescence analysis of U2OS cells using Anti-N6-methyladenosine/m6A Antibody (A309558). U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time. DAPI, 4', 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.
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