Rabbit polyclonal antibody to Na,K-ATPase alpha1 (Phospho-Ser23)
Specificity
The antibody detects endogenous level of Na,K-ATPase alpha1 only when phosphorylated at Serine 23.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 23 (K-K-S(p)-K-K) derived from Rat Na,K-ATPase alpha1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
113kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Rat brain tissue using Na,K-ATPase alpha1 (Phospho-Ser23) Antibody #12052.The lane on the left is treated with the antigen-specific peptide.
