Anti-NADH2 Antibody (A92937)

Name: Anti-NADH2 Antibody
Brand: Antibodies.com
SKU: A92937
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to NADH2 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NADH2 Antibody

Description

Rabbit polyclonal antibody to NADH2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 248-347 of human MT-ND2 (YP_003024027.1).

Sequence

LLSLGGLPPLTGFLPKWAIIEEFTKNNSLIIPTIMATITLLNLYFYLRLIYSTSITLLPMSNNVKMKWQFEHTKPTPFLPTLIALTTLLLPISPFMLMIL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

39 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

MT-ND2, MTND2, NADH dehydrogenase subunit 2, NADH-ubiquinone oxidoreductase chain 2, ND2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-NADH2 Antibody (A92937)

Western blot analysis of extracts of various cell lines, using Anti-NADH2 [9E12-1B3] Antibody (A92937) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Immunohistochemistry - Anti-NADH2 Antibody (A92937)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-NADH2 [9E12-1B3] Antibody (A92937) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-NADH2 Antibody (A92937)

Immunofluorescence analysis of HeLa cells using Anti-NADH2 [9E12-1B3] Antibody (A92937) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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