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Anti-NCF1/p47-phox Antibody (A11422)

Western Blot - Anti-NCF1/p47-phox Antibody (A11422) - Antibodies.com Western Blot - Anti-NCF1/p47-phox Antibody (A11422) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to NCF1/p47-phox for WB, ICC/IF and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-NCF1/p47-phox Antibody
Description
Rabbit polyclonal antibody to NCF1/p47-phox.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:100-1:500, ICC/IF: 1:50-1:100, IP: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human p47phox/NCF1 (NP_000256.4).
Sequence
MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
47 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-NCF1/p47-phox Antibody (A11422) - Antibodies.comEnlarge Image

Western Blot - Anti-NCF1/p47-phox Antibody (A11422)

Western blot analysis of various lysates, using Anti-NCF1/p47-phox Antibody (A11422) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-NCF1/p47-phox Antibody (A11422) - Antibodies.comEnlarge Image

Western Blot - Anti-NCF1/p47-phox Antibody (A11422)

Immunoprecipitation analysis of 200µg extracts of Raji cells using 3µg of Anti-NCF1/p47-phox Antibody (A11422). This Western blot was performed on the immunoprecipitate using Anti-NCF1/p47-phox Antibody (A11422) at a dilution of 1:1000.
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