Anti-NDUFB5 Antibody (A305728)

Name: Anti-NDUFB5 Antibody
Brand: Antibodies.com
SKU: A305728

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This product is discontinued

Anti-NDUFB5 Antibody (A305728) has been discontinued and is no longer available.

View all NDUFB5 Antibodies.

Specifications

Name

Anti-NDUFB5 Antibody

Description

Rabbit polyclonal antibody to NDUFB5.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-189 of human NDUFB5 (NP_002483.1).

Sequence

EIPEGYVPEHWEYYKHPISRWIARNFYDSPEKIYERTMAVLQIEAEKAELRVKELEVRKLMHVRGDGPWYYYETIDKELIDHSPKATPDN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CI-SGDH, Complex I-SGDH, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial, NADH-ubiquinone oxidoreductase SGDH subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-NDUFB5 Antibody (A305728)

Western blot analysis of extracts from normal (control) and NDUFB5 knockout (KO) HeLa cells, using Anti-NDUFB5 Antibody (A305728) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.