Anti-NDUFS3 Antibody [ARC1049] (A309182)

Name: Anti-NDUFS3 Antibody [ARC1049]
Brand: Antibodies.com
SKU: A309182
Price: 340.0 GBP
Availability: InStock

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This product is discontinued

Anti-NDUFS3 Antibody [ARC1049] (A309182) has been discontinued and is no longer available.

View all NDUFS3 Antibodies.

Specifications

Name

Anti-NDUFS3 Antibody [ARC1049]

Description

Rabbit monoclonal [ARC1049] antibody to NDUFS3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 165-264 of human NDUFS3 (O75489).

Sequence

WYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1049

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

28 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CI-30kD, Complex I-30kD, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH-ubiquinone oxidoreductase 30 kDa subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-NDUFS3 Antibody [ARC1049] (A309182)

Western blot analysis of extracts of various cell lines, using Anti-NDUFS3 Antibody (A309182) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-NDUFS3 Antibody [ARC1049] (A309182)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-NDUFS3 Antibody (A309182) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.