Mouse monoclonal [R2.525] antibody to Nectin 2 (PE).
Specificity
This antibody recognizes CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NIH/3T3 cells transfected with human Nectin-2.
Host
Mouse
Clonality
Monoclonal
Clone ID
R2.525
Isotype
IgG1
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of CACO-2 cells stained with Anti-Nectin 2 Antibody [R2.525] (PE) (A86336), (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from CACO-2 cells stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 5 µg/ml, same as CD112 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of CACO-2 cell suspension.
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